Tnt nitroreductase
Webb19 sep. 2024 · A nitroreductase encoded gene from an efficient nitro‐reducing bacterium Streptomyces mirabilis DUT001, named snr, was cloned and heterogeneously expressed in Escherichia coli. The purified... Webb1 apr. 2000 · Type I nitroreductase, purified from E. cloacae, oxidized NADPH with TNT or RDX as substrate. When expression of the E. cloacae type I nitroreductase gene was induced in an Escherichia coli...
Tnt nitroreductase
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Webb3 jan. 2024 · TNT reduction activity was 2.8-fold higher by NitB enzyme when NADH served as electron donor compared to NADPH. NitA enzyme showed K m values of 8.51 μM and … WebbTransgenic plants harboring bacterial genes, including pentaerythritol tetranitrate reductase, nitroreductase, or cytochrome P450, have become tolerant to TNT, and phytoremediation has been proposed for detoxifying nitroaromatic explosives. More examples of remediation using bacterial nitroreductase have been reviewed extensively …
Webb1 dec. 2001 · Nitroreductase catalyzes the reduction of TNT to hydroxyaminodinitrotoluene (HADNT), which is subsequently reduced to aminodinitrotoluene derivatives (ADNTs). Transgenic plants expressing nitroreductase show a striking increase in ability to tolerate, take up, and detoxify TNT. Webb1 mars 2000 · Many enteric bacteria express a type I oxygen-insensitive nitroreductase, which reduces nitro groups on many different nitroaromatic compounds under aerobic conditions. Enzymatic reduction of nitramines was also documented in enteric bacteria under anaerobic conditions. This study indicates that nitramine reduction in enteric …
WebbThe substrate specificity test revealed that the nitroreductase exhibited the highest enzyme activity for the TNT substrate of the nitroaromatic compounds tested in this … Webb1 feb. 2011 · Nitrite liberation was consistently found coincided with TNT disappearance from the medium. The disappearance of TNT was completely within 7days by the two isolates and/or their mixture. This...
Webb5 jan. 2008 · Expression and Characterization of the TNT Nitroreductase of Pseudomonas sp. HK-6 in Escherichia coli, Current Microbiology 10.1007/s00284-007-9093-5 DeepDyve DeepDyve Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team. Learn More →
Webb1 aug. 2005 · In this study we have identified the nitroreductase-family enzymes PnrA and PnrB from the TNT-degrading strain Pseudomonas putida. The enzyme encoded by the pnrA gene was expressed in Escherichia coli, purified to homogeneity and shown to be a flavoprotein that used 2 mol of NADPH to reduce 1 mol of 2,4,6-trinitrotoluene (TNT) to 4 … halal restaurant in pavilion klWebb2,4,6-trinitrotoluene (TNT) and the involvement of nitroreductase (NR) enzyme in TNT degradation in vetiver has been documented in our earlier studies. The present study was designed to optimize the parameters influencing NR enzyme-mediated TNT transformation in vetiver. The current study is the first report of detailed kinetic exami- halal restaurants in osaka japanWebb1 apr. 2024 · TNT is toxic to aquatic flora and other organisms and animals, including reptiles. TNT is classified as mutagenic, poisonous, and carcinogenic. Even at a very low concentration of 50 mg/L, TNT is lethal for microorganisms including Gram-positive bacteria and yeasts [ 7]. halal restaurants in kutaisiWebbAbstract. The preparation and characterization of an amperometric 2,4,6-trinitrotoluene (TNT) biosensor based on the surface immobilization of a maltose binding protein … halal restaurants in suttonWebb1 maj 2015 · E. coli nitroreductases Transformation pathway Structure-based mutagenesis 1. Introduction 2,4,6-Trinitrotoluene (TNT), the most widely used military explosive and widespread environmental contaminant, has caused serious health and environmental issues because of its acute toxicity and mutagenicity [1], [2]. halal ruokavalioWebbof the total nitroreductase activity within the three peaks is approximately equal in both E. coli and S. typhimurium, the major peak isolated during the purification of the E. cloacae nitroreductase represents 90% of the total activity recovered. Thus, it appears that TNT induces the expression of a specific nitroreductase. halal restaurant hillion mallWebb1 okt. 2008 · Here, we study the capacity of transgenic hybrid aspen (Populus tremula x tremuloides var. Etropole) which expresses the bacterial nitroreductase gene, pnrA, to tolerate and take-up greater amounts of the toxic and recalcitrant explosive, 2,4,6-trinitrotoluene (TNT) from contaminated waters and soil. halal rueil malmaison