Recognizing the reagent microbiome
Webbanalysis of the genomes of the microbes present, termed the microbiome. Increasingly, organs and tissues thought once to be sterile are shown to host commensal bacteria in the absence of infection, including the upper female reproductive tract and the placenta (Baker et al., 2024; Benner et al., 2024; Evans et al., 2016; Koedooder et al., 2024). WebbRecognizing the reagent microbiome A noticeable part of the microbiome literature, especially that working with low-biomass samples, is plagued by reagent contamination. …
Recognizing the reagent microbiome
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Webb14 apr. 2024 · The microbiome in a sample is represented by the relative abundances of all the single OTUs (i.e., ... de Goffau MC, Lager S, Salter SJ, Wagner J, Kronbichler A, Charnock-Jones DS, Peacock SJ, Smith GCS, Parkhill J. 2024. Recognizing the reagent microbiome. Nat Microbiol. 3(8):851–853. Crossref. PubMed. Google Scholar. Webb5 juli 2024 · Data regarding the in utero microbiome environment are based mainly on studies ... Turek EM et al. Reagent and laboratory contamination can critically impact sequence-based microbiome analyses. BMC Biol. 12(1), 87 (2014).Crossref, Medline, Google Scholar; 23. de Goffau MC, Lager S, Salter SJ et al. Recognizing the reagent …
Webb1 nov. 2024 · In this interview, Alan discusses his research interests, earlier studies of the ways contaminants can affect microbiome analyses, the excitement of experiments going well, and why science doesn’t need to be ... Salter SJ, Wagner J, Kronbichler A, Charnock-Jones DS, et al. Recognizing the reagent microbiome. Nat Microbiol. 2024;3(8 ... WebbRecognizing the reagent microbiome. Nature Microbiology;3:851-853. Gaccioli F, Sovio U, Cook E, Hund M, Charnock-Jones DS, Smith GCS. (2024). Screening for fetal growth restriction using ultrasound and the sFLT1:PlGF ratio in a prospective cohort study of nulliparous women.
Webb24. de Goffau MC, Lager S, Salter SJ, et al. Recognizing the reagent microbiome. Nat Microbiol 2024; 3:851–853. Cited Here Google Scholar; 25. Verstraelen H, Vilchez-Vargas R, Desimpel F, et al. Characterisation of the human uterine microbiome in nonpregnant women through deep sequencing of the V1-2 region of the 16S rRNA gene. WebbCitation 24 We identified shotgun reads positive for bacteria such as Bordetella bronchiseptica, Streptococcus suis, Staphylococcus aureus, Methylobacterium sp., Alteromonas mediterranea in all of the tested samples including reagent control and germ-free tissues suggesting they represent the reagent microbiome (Figure 2a-f).
Webb9 mars 2024 · The possibility that the microbiome from these low-biomass communities could be a contamination from other tissues and reagents was ... M. C. et al. …
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