Phic31 integrase

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August undefined, 2024

WebNational Center for Biotechnology Information Web• Developed phiC31 integrase system in Hydra to optimize transgenesis via site-specific recombination. • Designed DNA constructs for microinjections. • Mentored undergraduate students and ...

(PDF) Phage Integrases for Genome Editing - ResearchGate

WebINTRODUCTION: The phiC31 integrase system is a phage-derived system that offers the ability to integrate plasmid DNA into the chromosomes at a subset of endogenous preferred locations associated with robust gene expression. Recent progress highlights the unique advantages of this system for in vivo gene therapy and for use in stem cells. WebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide … scott bolden wife

The Application of Nanobody in CAR-T Therapy - MDPI

WebThe PhiC31 Integrase System Frequently used for gene therapy development and related applications Non-viral transgene delivery Single-step process Single-copy integration Preferential integration at active sites Delivery of inserts with no size constraints Easy generation of cell lines The PinPoint Targeted Integration System WebJan 1, 2024 · So far, only the phiC31 integrase has been shown to work efficiently in Parhyale (Kontarakis et al., 2011) and a version of the Raeppli clonal marker which is … WebPhiC31 integrase is a serine recombinase containing an N-terminal domain (NTD) that provides catalytic activity and a large C-terminal domain that controls which pair of DNA substrates is able to ... scott boland cricket family

phiC31 integrase for recombination-mediated single-copy …

Anti-Bacteriophage PhiC31 Integrase antibody (ab93248) - Abcam

WebpcDNA3.1 phiC31 was a gift from Konrad Basler (Addgene plasmid # 68310 ; http://n2t.net/addgene:68310 ; RRID:Addgene_68310) For your References section: An … WebMar 4, 2016 · Phage integrases are prokaryotic site-specific recombinases that perform precise cut-and-paste recombination between their short attB and attP recognition sequences. These enzymes work in cellular... scott boldtWebOct 2, 2024 · One possibility is to use the integrase of phage phiC31 (phiC31-int) which catalyzes efficient site-specific recombination … prename of bacon\u0027s spouse

"WebThe phiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage phiC31. The phiC31 integrase mediates recombination between two … " - Phic31 integrase

Phic31 integrase

WebOver the past decade, the integrase enzyme from phage phiC31 has proven to be a useful genome engineering tool in a wide variety of species, including mammalian cells. WebJan 14, 2024 · PhiC31 integrase-mediated transgenesis systems are based on the site-specific bacteriophage PhiC31 integrase which mediates sequence-directed, irreversible and highly efficient integration between a bacterial attachment site ( attB) and a phage attachment site ( attP ).

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Web加工所畜禽水产加工团队在《Food Bioscience》上发表高水平论文 2024.03.30; 经作所花卉团队在国际学术期刊《Biology》上发表月季抗病研究文章 2024.03.16; 加工所联合钟祥兴利公司等单位制定国内首个米茶类食品加工标准 2024.03.15; 经作所蔬菜团队在国际学术期刊《International Journal of Molecular Sciences》上发表 ... WebpUPD2 PhiC31 integrase (GB1561) was a gift from Diego Orzaez (Addgene plasmid # 160586 ; http://n2t.net/addgene:160586 ; RRID:Addgene_160586) For your References section: A memory switch for plant synthetic biology based on the phage varphiC31 integration system.

WebJul 25, 2024 · A master cell line, with the landing pad inserted correctly in the H11 locus, was established. This master cell line was used for site-specific, irreversible recombination, catalyzed by PhiC31 integrase. Using this system, an integration efficiency of 97.7% was achieved with green fluorescent protein (GFP) after selection. WebIsolated from the bacteriophage PhiC31, the PhiC31 integrase (frequently also written as: ΦC31 integrase) encodes a serine-type recombinase that mediates the sequence-specific …

WebThe Jump-In™ technology helps accelerate stable cell line development due to the targeted integration of genetic material into specific integration sites using PhiC31 integrase. … The mode integration/resolution and inversion (INT/RES and INV) depend on the orientation of recombinase target sites (RTS), among these pairs of attP and attB. Section C indicates, in a streamlined fashion, the way recombinase-mediated cassette exchange (RMCE) can be reached by synchronous double-reciprocal crossovers (rather than integration, followed by resolution).

Webbacteriophage phiC31. The phiC31 integrase mediates recombination between two 34 base pair sequences termed attachment sites (att), one found in the phage and the other in the …

WebFeb 4, 2024 · We generated an integrated phiC31 integrase expressing strain flanked by attP sites that serves as a landing pad for integration of transgenes by recombination … prenatal 19 with docusateWebMar 20, 2024 · The phiC31 integrase system, as a site-specific recombinase, could be a straightforward and efficient approach for this aim. According to the high potential of the application of phiC31 integrase system in stem cells, the present paper reviews previous studies, placing the focus on stem cell types and their application. scott bolduc marinehttp://www.hbaas.com/xumusuo/view/15912.html scott bolding oral surgeonWebφC31 integrase encoded by Streptomyces phage can mediate site-specific recombination between phage and host genomes. The recombination direction is generally considered to be accurately... prenancy medicaid greenville ncWebFinally, the serine integrase PhiC31 was also applied in the yeast Saccharomyces cerevisiae as a marker recovery tool, indicating the … scott bolden show cause orderWebpUPD2 PhiC31 integrase (GB1561) was a gift from Diego Orzaez (Addgene plasmid # 160586 ; http://n2t.net/addgene:160586 ; RRID:Addgene_160586) For your References … scott bolducWebJul 20, 2024 · We employed the PhiC31 integrase system to generate transgenic lines that permit overexpression of wild-type (WT) Drosophila TnT or the fly equivalent of the … prenancy tests reddin ca