Eea1 molecular weight
WebEarly endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane … WebCell Signaling Technology Inc wo2 mouse monoclonal Wo2 Mouse Monoclonal, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.
Eea1 molecular weight
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WebThe expected molecular weight of EEA1 (on the left-hand side) and molecular weight standards (on the right-hand side) are shown in the figure. (B) Fusion between early … WebPrimary Anti-EEA1 antibody - Early Endosome Marker (ab2900) View more associated products Description Product name Recombinant Human EEA1 protein Biological activity …
Web1/2000 - 1/10000. Predicted molecular weight: 162 kDa. Notes; IP Use at 2-5 µg/mg of lysate. WB 1/2000 - 1/10000. Predicted molecular weight: 162 kDa. Target. Function. ... EEA1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using … WebJun 2, 1995 · EEA1 is a hydrophilic peripheral membrane protein present in cytosol and membrane fractions. It partitions in the aqueous phase after Triton X-114 solubilization …
WebJun 2, 1995 · We have cloned a human cDNA encoding an evolutionarily conserved 180-kDa protein on early endosomes named EEA1 (Early Endosome Antigen1). EEA1 is associated with early endosomes since it co-localizes by immunofluorescence with the transferrin receptor and with Rab5 but not with Rab7. Immunoelectron microscopy… WebOct 13, 2024 · EEA1 is an early endosomal Rab5 effector protein that has been implicated in the docking of incoming endocytic vesicles before fusion with early endosomes. Because of the presence of complex endosomal …
WebEarly endosome antigen 1 (EEA1) is a 180 kDa hydrophilic peripheral membrane protein present in cytosol and membrane fractions. Immunofluorescence studies show that …
WebApr 10, 2024 · The mode of transport depends on the physical and chemical properties of the substances to be transported, including molecular weight, lipid solubility, plasma protein binding, and cerebral blood flow. Passive transport is limited to small-sized molecules that have sufficient lipid solubility such as gaseous oxygen and carbon dioxide … mild walnut allergyWebJun 5, 2012 · Different effectors of the endocytic pathways, as for example EEA1 and Rab5, are regulated through phosphorylation by p38. ... The capsule of B. anthracis is produced as high-molecular weight form (>100 kDa), which is first polymerized on the bacterial cell surface in vivo and subsequently degraded to the lower-molecular weight capsule (<14 … mild warmWebMay 1, 2024 · Early endosomes, regarded as the main sorting station on endocytic pathway, are characterized by high frequency of homotypic fusions mediated by tethering protein EEA1. Despite intensive investigations, biogenesis of endosomes, boundaries between early and late endosomes, and process of cargo transition though them remain obscure. … mild ware 鍋WebEEA1 (G-4) is a mouse monoclonal antibody raised against amino acids 1-300 mapping at the N-terminus of EEA1 of human origin. PRODUCT 1. Each vial contains 200 µg IgG 1 … new year\u0027s snacksWebEEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting … new year\u0027s smash 2022WebApr 10, 2024 · The lower molecular weight protein band, detected by the anti-human Alix antibody in bovine and swine cell lysates, may represent unspecific staining. ... like the early endosome-associated protein 1 (EEA1), recycling endosome-decorating Rab11, the late endosome marker Rab7, and the endolysosomal LAMP1 (lysosomal-associated … mild warningWebView Rabbit Polyclonal anti-EEA1 Antibody (NBP1-30914). Validated Applications: WB, ICC/IF, IHC, IHC-P, IM. Validated Species: Human, Mouse, Rat + more. ... The observed … mild warm luft